Yes. You can easily split payments across two or more cards using CardUp. The payments will appear as separate transactions to your recipient on his/her statement but rest assured that the funds will be credited on time and together if specified. Furthermore, you will also be able to add a personalised payment reference to reflect on the bank transfer.
An example of what your recipient might see on his bank statement:
CardUp.co: Jon Snow - Rental November1
CardUp.co: Jon Snow - Rental November2